Coding

Part:BBa_K1882006:Design

Designed by: Milena Krach   Group: iGEM16_Tuebingen   (2016-10-14)


Fructose Transporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 523
    Illegal NheI site found at 838
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 9
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1678


Design Notes

The codon usage of this part was optimized to balance DNA synthesis and expression in l.Johnsonii. Therefore longterm and shortterm repeats had to modified and biobrick restriction sites were removed.The part was modified for RFC10 at the prefix and suffix of the part.


Source

Lactobacillus johnsonii ATCC33200, NCBI reference number: NZ_AZCY01000006.1

References